iTools是一个非常好用的工具包，目前有以下的功能：
1 Fatools main deals with Fasta format;
2 FqTools main deals with Fastq format;
3 SOAPtools deals with SOAP format which is the result of SOAP[3].
4 CNSTools deals with cns format which is the result of soapsnp[4].
5 XamTools deals with sam/bam format which is the result of bwa[5]
6 Gfftools deals with gff format which is the genome annotation file.
7 Formatools is the converter of some Custom format file.
8 Gametools is the package for some classics games
9 Filetools is the tools for file with fixed format
10 Vartools Tools For SOAP Variant
11 Othrtools LDDecay etc
第一步，安装软件
wget https://raw.githubusercontent.com/BGI-shenzhen/Reseqtools/master/iTools_Code20180520.tar.gz
tar -zxvf iTools_Code20180520.tar.gz
cd iTools_Code
chmod 775 iTools
运行
./iTools -h
即可获得软件使用提示。
第二步，对fastq文件进行质量值统计
iTools Fqtools stat -InFq xxx.fastq -OutStat results.txt
可以添加多几个-InFq参数来统计多个fastq文件，也可以通过-InFqList参数来统计List内的文件。
输出结果是这样的：
